Difference between revisions of "Part:BBa K4407002:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | PFOR, the main enzyme that catalyzes the reaction of pyruvate to acetyl CoA, in C. tyrobutyricum is inactivated in the presence of oxygen. Therefore, the aceE gene from the facultative anaerobic E. coli bacteria that encodes a component of the PDH complex was used. PDH could be enacted in the presence of oxygen to achieve the same metabolic function as PFOR. | |
| Line 13: | Line 13: | ||
===Source=== | ===Source=== | ||
| − | + | The source of the part will be its sequence retrieved from GenBank. | |
===References=== | ===References=== | ||
Latest revision as of 08:27, 8 October 2022
aceE，Alternate metabolic pathway
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1067
Illegal BglII site found at 1475
Illegal BamHI site found at 27 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2097
Illegal AgeI site found at 808 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
PFOR, the main enzyme that catalyzes the reaction of pyruvate to acetyl CoA, in C. tyrobutyricum is inactivated in the presence of oxygen. Therefore, the aceE gene from the facultative anaerobic E. coli bacteria that encodes a component of the PDH complex was used. PDH could be enacted in the presence of oxygen to achieve the same metabolic function as PFOR.
Source
The source of the part will be its sequence retrieved from GenBank.