Difference between revisions of "Part:BBa K4407002"
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<partinfo>BBa_K4407002 short</partinfo> | <partinfo>BBa_K4407002 short</partinfo> | ||
| − | + | This part contains the coding region of the aceE protein. aceE gene is a pyruvate dehydrogenase (PDH) gene from E. coli MG1655. aceE protein is a component of the PDH complex and catalyzes the conversion of pyruvate to acetyl-CoA and CO2. It can function well in aerobic environment. In our project, we inserted aceE gene into Clostridium tyrobutyricum (C. tyrobutyricum) to make the bacteria facultative anaerobic. aceE protein expressed in C. tyrobutyricum can carry out the function of PFOR which is inactivated under aerobic conditions and maintain the energy metabolism of the bacteria in aerobic environment. | |
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Latest revision as of 08:26, 8 October 2022
aceE，Alternate metabolic pathway
This part contains the coding region of the aceE protein. aceE gene is a pyruvate dehydrogenase (PDH) gene from E. coli MG1655. aceE protein is a component of the PDH complex and catalyzes the conversion of pyruvate to acetyl-CoA and CO2. It can function well in aerobic environment. In our project, we inserted aceE gene into Clostridium tyrobutyricum (C. tyrobutyricum) to make the bacteria facultative anaerobic. aceE protein expressed in C. tyrobutyricum can carry out the function of PFOR which is inactivated under aerobic conditions and maintain the energy metabolism of the bacteria in aerobic environment.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1067
Illegal BglII site found at 1475
Illegal BamHI site found at 27 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2097
Illegal AgeI site found at 808 - 1000COMPATIBLE WITH RFC[1000]