Difference between revisions of "Part:BBa K4197016"
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<h2>Introduction</h2> | <h2>Introduction</h2> | ||
| − | <p>This part is composed of the gene coding for the allergen of cashew Ana o 3 (NCBI: <a "https://www.ncbi.nlm.nih.gov/protein/AAL91665.1/">AAL91665.1</a>). The cashew allergy prevalence is | + | <p>This part is composed of the gene coding for the allergen of cashew Ana o 3 (NCBI: <a "https://www.ncbi.nlm.nih.gov/protein/AAL91665.1/">AAL91665.1</a>). The cashew allergy prevalence is higher than 0.08% (Van der Valk and al. 2014) in the US countries and Ana o 3 binds specific antibodies of 100% of the patients with cashew allergy (Sato and al. 2019). Ana o 3 have already been expressed in <i>E. coli </i>and was able to bind the IgE of patient with cashew allergie (Robotham and al. 2005).</p> |
<h2>Construction</h2> | <h2>Construction</h2> | ||
| − | <p>The objective of the INSA-UPS 2022 team was to display Ana o 3 on the surface of <i>E. coli</i> so the ordered sequence was merged to OmpA membrane | + | <p>The objective of the INSA-UPS 2022 team was to display Ana o 3 on the surface of <i>E. coli</i> so the ordered sequence was merged to OmpA membrane lipoprotein (see part <a href="https://parts.igem.org/Part:BBa_K4197007">BBa_K4197007</a>).</p> |
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<p>Other parts to display allergens:<br> | <p>Other parts to display allergens:<br> | ||
- <a href="https://parts.igem.org/Part:BBa_K4197008"> OmpA_Ara h 2</a> <br> | - <a href="https://parts.igem.org/Part:BBa_K4197008"> OmpA_Ara h 2</a> <br> | ||
| − | - <a href="https://parts.igem.org/Part: | + | - <a href="https://parts.igem.org/Part:BBa_K4197006"> OmpA_Der p 2</a> <br> |
| − | - <a href="https://parts.igem.org/Part: | + | - <a href="https://parts.igem.org/Part:BBa_K4197009"> OmpA_Gal d 2</a> <br> |
</p> | </p> | ||
Latest revision as of 07:51, 8 October 2022
Gene coding for Ana o 3
Gene coding for the cashew allergen called Ana o 3.
Introduction
This part is composed of the gene coding for the allergen of cashew Ana o 3 (NCBI: AAL91665.1). The cashew allergy prevalence is higher than 0.08% (Van der Valk and al. 2014) in the US countries and Ana o 3 binds specific antibodies of 100% of the patients with cashew allergy (Sato and al. 2019). Ana o 3 have already been expressed in E. coli and was able to bind the IgE of patient with cashew allergie (Robotham and al. 2005).
Construction
The objective of the INSA-UPS 2022 team was to display Ana o 3 on the surface of E. coli so the ordered sequence was merged to OmpA membrane lipoprotein (see part BBa_K4197007).
References
More information about the project for which the part was created: DAISY (INSA-UPS 2022)
Other parts to display allergens:
- OmpA_Ara h 2
- OmpA_Der p 2
- OmpA_Gal d 2
- Van der Valk, J. P. M., J. Dubois, A. E., Gerth van Wijk, R., Wichers, H. J., de Jong, N. W. (2014). Systematic review on cashew nut allergy. Allergy. 69(6), 692–698. doi:10.1111/all.12401
- Sato, S., Movérare, R., Ohya, Y., Ito, K., Nagao, M., Borres, M. P., & Ebisawa, M. (2019). Ana o 3–specific IgE is a predictive marker for cashew oral food challenge failure. The Journal of Allergy and Clinical Immunology : In Practice, 7(8), 2909–2911.e4. https://doi.org/10.1016/j.jaip.2019.04.049
- Robotham, J. M., Wang, F., Seamon, V., Teuber, S. S., Sathe, S. K., Sampson, H. A., Beyer, K., Seavy, M., & Roux, K. H. (2005). Ana o 3, an important cashew nut (Anacardium occidentale L.) allergen of the 2S albumin family. Journal of Allergy and Clinical Immunology, 115(6), 1284–1290.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 130