Difference between revisions of "Part:BBa K4413993"
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gabY is the gene used for promotes the combination of PQQ coenzyme and GDH, increases the effective concentration of the holoenzymes, enhances GDH’s catalytic efficiency, and we insert RiboJ between promoter and RBS to increase Translation efficiency.With the over-expression of gabY, it can decrease the pH to a greater extent and promote the dissolution of phosphate. | gabY is the gene used for promotes the combination of PQQ coenzyme and GDH, increases the effective concentration of the holoenzymes, enhances GDH’s catalytic efficiency, and we insert RiboJ between promoter and RBS to increase Translation efficiency.With the over-expression of gabY, it can decrease the pH to a greater extent and promote the dissolution of phosphate. | ||
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Revision as of 07:43, 8 October 2022
PGroES-RiboJ-DrRBS-gabY
gabY is the gene used for promotes the combination of PQQ coenzyme and GDH, increases the effective concentration of the holoenzymes, enhances GDH’s catalytic efficiency, and we insert RiboJ between promoter and RBS to increase Translation efficiency.With the over-expression of gabY, it can decrease the pH to a greater extent and promote the dissolution of phosphate.
11
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 476
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 190
Illegal NgoMIV site found at 390
Illegal NgoMIV site found at 482
Illegal NgoMIV site found at 648 - 1000COMPATIBLE WITH RFC[1000]