Difference between revisions of "Part:BBa K4425013"

Latest revision as of 07:32, 8 October 2022

pET28a-BGL

We construct plasmid pET28a-BGL to express BGL to convert indican to indoxyl.

Usage and Biology

BGL was assemble with pET28a backbone to construct pET28a-BGL. BGL is induced to express to convert soluble indican to indoxyl, and the lattet can turn to indigo (or tyrian purple) by spontaneous oxidation

Source

BGL comes from P. tinctorium

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 190
Illegal EcoRI site found at 196
Illegal XbaI site found at 47
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 190
Illegal EcoRI site found at 196
Illegal NheI site found at 151
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 190
Illegal EcoRI site found at 196
Illegal BamHI site found at 184
Illegal XhoI site found at 1618
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 190
Illegal EcoRI site found at 196
Illegal XbaI site found at 47
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 190
Illegal EcoRI site found at 196
Illegal XbaI site found at 47
Illegal AgeI site found at 529
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4425013 short</partinfo>
-a
+We construct plasmid pET28a-BGL to express BGL to convert indican to indoxyl.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+BGL was assemble with pET28a backbone to construct pET28a-BGL. BGL is induced to express to convert soluble indican to indoxyl, and the lattet can turn to indigo (or tyrian purple) by spontaneous oxidation
+==Source==
+BGL comes from P. tinctorium
-<!-- -->
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K4425013 SequenceAndFeatures</partinfo>