Difference between revisions of "Part:BBa K4144010"

 
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<partinfo>BBa_K4144010 short</partinfo>
 
<partinfo>BBa_K4144010 short</partinfo>
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===Usage and Biology===
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Linkers can be classified into three groups: flexible, rigid and cleavable. Flexible linkers are generally composed of small, non-polar or polar residues such as Gly, Ser and Thr. The most common is the (Gly4Ser)n linker (Gly–Gly–Gly–Gly–Ser)n, where n indicates the number of repeats of the motif. Polyglycine linkers have also been evaluated, but the addition of a polar residue such as serine can reduce linker–protein interactions and preserve protein function. Due to their flexibility, these linkers are unstructured and thus provided limited domain separation in a previous study.<br>
  
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Improved recombined SAMe transporter opPet8p.
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We WHU-China plan to use such linker to contact opPet8p and sfGFP in order to avoid the opPet8p membrane localization disruption by adding a sfGFP tag.
  
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===Usage and Biology===
 
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4144010 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4144010 SequenceAndFeatures</partinfo>

Revision as of 07:31, 8 October 2022


Improved recombined SAMe transporter opPet8p fused with linker

Usage and Biology

Linkers can be classified into three groups: flexible, rigid and cleavable. Flexible linkers are generally composed of small, non-polar or polar residues such as Gly, Ser and Thr. The most common is the (Gly4Ser)n linker (Gly–Gly–Gly–Gly–Ser)n, where n indicates the number of repeats of the motif. Polyglycine linkers have also been evaluated, but the addition of a polar residue such as serine can reduce linker–protein interactions and preserve protein function. Due to their flexibility, these linkers are unstructured and thus provided limited domain separation in a previous study.

We WHU-China plan to use such linker to contact opPet8p and sfGFP in order to avoid the opPet8p membrane localization disruption by adding a sfGFP tag.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 520
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 390
    Illegal BamHI site found at 1018
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1642
  • 1000
    COMPATIBLE WITH RFC[1000]