Difference between revisions of "Part:BBa K4144009"

Revision as of 07:28, 8 October 2022

A linker between protein of interest and tag

A linker between protein of interest and tag.

Usage and Biology

Linkers can be classified into three groups: flexible, rigid and cleavable. Flexible linkers are generally composed of small, non-polar or polar residues such as Gly, Ser and Thr. The most common is the (Gly4Ser)n linker (Gly–Gly–Gly–Gly–Ser)n, where n indicates the number of repeats of the motif. Polyglycine linkers have also been evaluated, but the addition of a polar residue such as serine can reduce linker–protein interactions and preserve protein function. Due to their flexibility, these linkers are unstructured and thus provided limited domain separation in a previous study.

Reference

[1] Mart Krupovicˇ and Dennis H. Bamford. Holin of bacteriophage lambda: structural insights into a membrane lesion. Molecular Microbiology(2008)69(4), 781–783.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 A linker between protein of interest and tag.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+Linkers can be classified into three groups: flexible, rigid and cleavable. Flexible linkers are generally composed of small, non-polar or polar residues such as Gly, Ser and Thr. The most common is the (Gly4Ser)n linker (Gly–Gly–Gly–Gly–Ser)n, where n indicates the number of repeats of the motif. Polyglycine linkers have also been evaluated, but the addition of a polar residue such as serine can reduce linker–protein interactions and preserve protein function. Due to their flexibility, these linkers are unstructured and thus provided limited domain separation in a previous study.<br>
+==Reference==
+[1] Mart Krupovicˇ and Dennis H. Bamford. Holin of bacteriophage lambda: structural insights into a membrane lesion. Molecular Microbiology(2008)69(4), 781–783.
 <!-- -->