Difference between revisions of "Part:BBa K4425010"

 
 
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<partinfo>BBa_K4425010 short</partinfo>
 
<partinfo>BBa_K4425010 short</partinfo>
  
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a
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Express Taq polymerase when co-transform with trp repressor that can bind to its ligand.
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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Active trp repressors with its ligand can induce pPOS to express Taq polymerase
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4425010 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4425010 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K4425010 parameters</partinfo>
 
<partinfo>BBa_K4425010 parameters</partinfo>
 
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==Reference==
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Ellefson, J. W., Ledbetter, M. P., & Ellington, A. D. Directed evolution of a synthetic phylogeny of programmable Trp repressors. Nature chemical biology, 2018, 14(4), 361–367.

Latest revision as of 07:19, 8 October 2022


pPOS

Express Taq polymerase when co-transform with trp repressor that can bind to its ligand.


Usage and Biology

Active trp repressors with its ligand can induce pPOS to express Taq polymerase

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 967
    Illegal XbaI site found at 715
    Illegal XbaI site found at 959
    Illegal PstI site found at 2564
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 967
    Illegal NheI site found at 3010
    Illegal PstI site found at 2564
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 967
    Illegal BglII site found at 718
    Illegal BglII site found at 956
    Illegal BamHI site found at 507
    Illegal BamHI site found at 2745
    Illegal XhoI site found at 1298
    Illegal XhoI site found at 2375
    Illegal XhoI site found at 3332
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 967
    Illegal XbaI site found at 715
    Illegal XbaI site found at 959
    Illegal PstI site found at 2564
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 967
    Illegal XbaI site found at 715
    Illegal XbaI site found at 959
    Illegal PstI site found at 2564
    Illegal NgoMIV site found at 2399
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2888
    Illegal BsaI.rc site found at 3128
    Illegal SapI site found at 2200
    Illegal SapI site found at 3268
    Illegal SapI.rc site found at 2590
    Illegal SapI.rc site found at 3178


Reference

Ellefson, J. W., Ledbetter, M. P., & Ellington, A. D. Directed evolution of a synthetic phylogeny of programmable Trp repressors. Nature chemical biology, 2018, 14(4), 361–367.