Difference between revisions of "Part:BBa K4425008"

 
 
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<partinfo>BBa_K4425008 short</partinfo>
 
<partinfo>BBa_K4425008 short</partinfo>
  
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express trp repressor
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Express muted trp repressor when induced by IPTG
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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pTrpR is induced to express muted trp repressors. Active trp repressors with its ligand can induce pPOS to express Taq polymerase and inactive trp repressors or trp repressor without its ligand can induce pNEG to express Taq polymerase.
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4425008 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4425008 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K4425008 parameters</partinfo>
 
<partinfo>BBa_K4425008 parameters</partinfo>
 
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==Reference==
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Ellefson, J. W., Ledbetter, M. P., & Ellington, A. D. Directed evolution of a synthetic phylogeny of programmable Trp repressors. Nature chemical biology, 2018, 14(4), 361–367.

Latest revision as of 07:04, 8 October 2022


lacUV5-TrpR1

Express muted trp repressor when induced by IPTG


Usage and Biology

pTrpR is induced to express muted trp repressors. Active trp repressors with its ligand can induce pPOS to express Taq polymerase and inactive trp repressors or trp repressor without its ligand can induce pNEG to express Taq polymerase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 192
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 19
    Illegal SapI.rc site found at 512


Reference

Ellefson, J. W., Ledbetter, M. P., & Ellington, A. D. Directed evolution of a synthetic phylogeny of programmable Trp repressors. Nature chemical biology, 2018, 14(4), 361–367.