Difference between revisions of "Part:BBa K4425007"

 
 
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<partinfo>BBa_K4425007 short</partinfo>
 
<partinfo>BBa_K4425007 short</partinfo>
  
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inverter based on &#955; repressor
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An inverter based on &#955; repressor and lambda promoter
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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The inverter is assembled in pPOS. when trp repressor bind to trp, the expression of lambda repressor will be repressed, and the promoter expression of the downstream lambda promoter will be excited to produce more Taq polymerase.
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==Source==
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lambda repressor is from bacteriophage lambda and trp operator is generated by Ellefson et al.
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4425007 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4425007 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K4425007 parameters</partinfo>
 
<partinfo>BBa_K4425007 parameters</partinfo>
 
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==Reference==
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Ellefson, J. W., Ledbetter, M. P., & Ellington, A. D. Directed evolution of a synthetic phylogeny of programmable Trp repressors. Nature chemical biology, 2018, 14(4), 361–367.

Latest revision as of 06:47, 8 October 2022


inverter based on λ repressor

An inverter based on λ repressor and lambda promoter


Usage and Biology

The inverter is assembled in pPOS. when trp repressor bind to trp, the expression of lambda repressor will be repressed, and the promoter expression of the downstream lambda promoter will be excited to produce more Taq polymerase.

Source

lambda repressor is from bacteriophage lambda and trp operator is generated by Ellefson et al.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 715
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 718
    Illegal BamHI site found at 507
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 715
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 715
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

Ellefson, J. W., Ledbetter, M. P., & Ellington, A. D. Directed evolution of a synthetic phylogeny of programmable Trp repressors. Nature chemical biology, 2018, 14(4), 361–367.