Difference between revisions of "Part:BBa K4425006"

 
 
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<partinfo>BBa_K4425006 short</partinfo>
 
<partinfo>BBa_K4425006 short</partinfo>
  
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protein expression
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Co-express of glucosyltransferase PtUGT and monooxygenase FMO
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===Usage and Biology===
 
===Usage and Biology===
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We construct plasmid pET28a-FMO-PtUGT to co-express PtUGT and FMO to convert indole or 6-Br-indole to indican or 6-Br-indican respectively.
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==Source==
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BBa K4425006 is composited by part BBa_K4425002 from <i>P. tinctorium<i/> and part BBa_K1131000 from <i>Methylophaga aminisulfidivorans</i>
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4425006 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4425006 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K4425006 parameters</partinfo>
 
<partinfo>BBa_K4425006 parameters</partinfo>
 
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==Reference==
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Hsu, T. M., Welner, D. H., Russ, Z. N., Cervantes, B., Prathuri, R. L., Adams, P. D., & Dueber, J. E. Employing a biochemical protecting group for a sustainable indigo dyeing strategy. Nature chemical biology, 2018, 14(3), 256–261.

Latest revision as of 06:31, 8 October 2022


pET28a-RBS-PtUGT-RBS-FMO

Co-express of glucosyltransferase PtUGT and monooxygenase FMO


Usage and Biology

We construct plasmid pET28a-FMO-PtUGT to co-express PtUGT and FMO to convert indole or 6-Br-indole to indican or 6-Br-indican respectively.

Source

BBa K4425006 is composited by part BBa_K4425002 from P. tinctorium<i/> and part BBa_K1131000 from <i>Methylophaga aminisulfidivorans

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1427
    Illegal BamHI site found at 1398
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2318
    Illegal AgeI site found at 2525
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1916


Reference

Hsu, T. M., Welner, D. H., Russ, Z. N., Cervantes, B., Prathuri, R. L., Adams, P. D., & Dueber, J. E. Employing a biochemical protecting group for a sustainable indigo dyeing strategy. Nature chemical biology, 2018, 14(3), 256–261.