Difference between revisions of "Part:BBa K4199002"

Revision as of 06:06, 8 October 2022

HrtR The interaction of heme iron with HrtR is non-covalent, six-coordinated, and involves two histidines, HIS-72 and HIS-149 HrtR specifically binding to the 15-NT palindrome sequence in the hrtRBA promoter region, which is required for hrtRBA inhibition Hrtr-dna binding is abolished by heme addition, which activates the expression of the HRTB-HRTA (HrtBA) transporter in vitro and in vivo. The use of HrtR as an intracellular heme sensor appears to be conserve in many symbiotic bacteria.[1]

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 249
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 249
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 249
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K4199002 short</partinfo>
-A transcription regulator(A heme binding protein)
+The interaction of heme iron with HrtR is non-covalent, six-coordinated, and involves two histidines, HIS-72 and HIS-149 HrtR specifically binding to the 15-NT palindrome sequence in the hrtRBA promoter region, which is required for hrtRBA inhibition Hrtr-dna binding is abolished by heme addition, which activates the expression of the HRTB-HRTA (HrtBA) transporter in vitro and in vivo. The use of HrtR as an intracellular heme sensor appears to be conserve in many symbiotic bacteria.[1]
@@ Line 8: / Line 8: @@
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
-The interaction of heme iron with HrtR is non-covalent, six-coordinated, and involves two histidines, HIS-72 and HIS-149 HrtR specifically binding to the 15-NT palindrome sequence in the hrtRBA promoter region, which is required for hrtRBA inhibition Hrtr-dna binding is abolished by heme addition, which activates the expression of the HRTB-HRTA (HrtBA) transporter in vitro and in vivo. The use of HrtR as an intracellular heme sensor appears to be conserve in many symbiotic bacteria.[1]