Difference between revisions of "Part:BBa K228004"
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Note: | Note: | ||
There is no termoinator downstream of the NahR coding sequence, but it has no effect on parts that are in the same direction as the pSal promoter. However, if coding gene is placed upstream of this part and in the same direction as NahR, it may cause unwanted expression. | There is no termoinator downstream of the NahR coding sequence, but it has no effect on parts that are in the same direction as the pSal promoter. However, if coding gene is placed upstream of this part and in the same direction as NahR, it may cause unwanted expression. | ||
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| + | This promoter is then placed upstream of a GFP gene to get it induction data. We use flowcytometry to test the fluorescence at different concentrations of the inducer salicylate. | ||
| + | The Induction curve is shown below: | ||
| + | |||
| + | [[Image:PKU_Salicylate.jpg|600px]] | ||
Revision as of 15:24, 20 September 2009
NahR( reverse) - salicylate promoter
This is a salicylate inducible promoter part. The activator protein is right upstream but in a opposite direction of the regulated promoter. In the presence of salicylate, the promoter can be activated.
Note: There is no termoinator downstream of the NahR coding sequence, but it has no effect on parts that are in the same direction as the pSal promoter. However, if coding gene is placed upstream of this part and in the same direction as NahR, it may cause unwanted expression.
This promoter is then placed upstream of a GFP gene to get it induction data. We use flowcytometry to test the fluorescence at different concentrations of the inducer salicylate. The Induction curve is shown below:
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 576
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 408
- 1000COMPATIBLE WITH RFC[1000]