Difference between revisions of "Part:BBa K4137012:Design"
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<partinfo>BBa_K4137012 short</partinfo>
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<partinfo>BBa_K4137012 SequenceAndFeatures</partinfo>
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===Design Notes===
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We chose to use p_mles, the mleR regulatory site as mleR can be induced by malate acid which surrounds the tree roots. CcdA is the antitoxin of the toxin Ccdb which kills chi18h8 when the probiotic isn’t near the roots of the banana tree. The Myc tag is used for protein quantification and is the most commonly used purification his-tag.
 
We chose to use p_mles, the mleR regulatory site as mleR can be induced by malate acid which surrounds the tree roots. CcdA is the antitoxin of the toxin Ccdb which kills chi18h8 when the probiotic isn’t near the roots of the banana tree. The Myc tag is used for protein quantification and is the most commonly used purification his-tag.
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===Source===
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https://www.ncbi.nlm.nih.gov/nuccore/X75982.1
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https://www.snapgene.com/resources/plasmid-files/?set=pet_and_duet_vectors_(novagen)&plasmid=pET-28a(%2B)
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https://www.ncbi.nlm.nih.gov/nuccore/U51588.1
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===References===

Revision as of 03:25, 8 October 2022

CcdA antitoxin secretion pathway


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We chose to use p_mles, the mleR regulatory site as mleR can be induced by malate acid which surrounds the tree roots. CcdA is the antitoxin of the toxin Ccdb which kills chi18h8 when the probiotic isn’t near the roots of the banana tree. The Myc tag is used for protein quantification and is the most commonly used purification his-tag.

Source

https://www.ncbi.nlm.nih.gov/nuccore/X75982.1 https://www.snapgene.com/resources/plasmid-files/?set=pet_and_duet_vectors_(novagen)&plasmid=pET-28a(%2B) https://www.ncbi.nlm.nih.gov/nuccore/U51588.1

References