Difference between revisions of "Part:BBa K4137014"
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| − | + | The mleR regulatory site which comes from p_mles binds with the RBS and CcdA, the antitoxin that will bind with CcdB when induced by malate acid and detoxifies it, the purification tag 6xHis, and the terminator B1006 (BBa_B1006). This sequence codes for CcdA, the antitoxin which assures chi18h8’s production around the plant roots. The second half contains a T7 promoter, a RBS, a mleR coding complex, a mleR purification tag—6xHis, and a terminator B1006. This half produces and purifies mleR, the regulator of CcdA, which produces when malate acid is present. The construct as a whole codes for Ccda regulated by mleR when malate acid is presented. | |
Revision as of 03:06, 8 October 2022
The mleR regulatory site which comes from p_mles binds with the RBS and CcdA, the antitoxin that will bind with CcdB when induced by malate acid and detoxifies it, the purification tag 6xHis, and the terminator B1006 (BBa_B1006). This sequence codes for CcdA, the antitoxin which assures chi18h8’s production around the plant roots. The second half contains a T7 promoter, a RBS, a mleR coding complex, a mleR purification tag—6xHis, and a terminator B1006. This half produces and purifies mleR, the regulator of CcdA, which produces when malate acid is present. The construct as a whole codes for Ccda regulated by mleR when malate acid is presented.