Difference between revisions of "Part:BBa K4137007"
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<partinfo>BBa_K4137007 short</partinfo> | <partinfo>BBa_K4137007 short</partinfo> | ||
| − | This part consists of a pLac promoter, RBS, a ccdB-Myc coding complex, and a terminator (BBa_B1006). This part produces a constant expression of ccdB toxin under the LB medium, inhibiting the GyrA subunit of DNA gyrase and prevents negative supercoiling of DNA. An accumulation of ccdB without sufficient suppression by antitoxin ccdA leads to risks in host viability. | + | This part consists of a pLac promoter, RBS (AAGGAG), a ccdB-Myc coding complex, and a terminator (BBa_B1006). This part produces a constant expression of ccdB toxin under the LB medium, inhibiting the GyrA subunit of DNA gyrase and prevents negative supercoiling of DNA. An accumulation of ccdB without sufficient suppression by antitoxin ccdA leads to risks in host viability. |
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Revision as of 02:41, 8 October 2022
pLac+RBS+CcdB-Myc+B1006
This part consists of a pLac promoter, RBS (AAGGAG), a ccdB-Myc coding complex, and a terminator (BBa_B1006). This part produces a constant expression of ccdB toxin under the LB medium, inhibiting the GyrA subunit of DNA gyrase and prevents negative supercoiling of DNA. An accumulation of ccdB without sufficient suppression by antitoxin ccdA leads to risks in host viability.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 271