Difference between revisions of "Part:BBa K4137009"

Revision as of 02:34, 8 October 2022

T7+RBS+YebF-GS Linker-chi18h8-6xHis+B1006

This part consists of a T7 strong promoter, RBS, a motor protein sequence yebF (BBa_K2922002), a GS Linker (BBa_J18921), a Chi19H8 coding sequence, a 6xHis tag (GTGx6), and a terminator (BBa_B1006). This part synthesizes and secretes a novel bacterial chitinase Chi18H8, which can be released extracellularly through e.coli Sec pathway (SecB-SecA-SecYEG, OmpF). This system continuously promotes the overexpression of Chi18H8 to lyse the chitin cell wall of the fungus fusarium oxysporum f.sp cubense, inhibiting fungal proliferation.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1523
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1036
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 583
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4137009 short</partinfo>
-This part consists of a T7 strong promoter, RBS, a motor protein sequence yebF (BBa_K2922002), a GS Linker (BBa_J18921), a Chi19H8 coding sequence, a 6xHis tag (), and a terminator (BBa_B1006). This part synthesizes and secretes a novel bacterial chitinase Chi18H8, which can be released extracellularly through e.coli Sec pathway (SecB-SecA-SecYEG, OmpF). This system continuously promotes the overexpression of Chi18H8 to lyse the chitin cell wall of the fungus fusarium oxysporum f.sp cubense, inhibiting fungal proliferation.
+This part consists of a T7 strong promoter, RBS, a motor protein sequence yebF (BBa_K2922002), a GS Linker (BBa_J18921), a Chi19H8 coding sequence, a 6xHis tag (GTGx6), and a terminator (BBa_B1006). This part synthesizes and secretes a novel bacterial chitinase Chi18H8, which can be released extracellularly through e.coli Sec pathway (SecB-SecA-SecYEG, OmpF). This system continuously promotes the overexpression of Chi18H8 to lyse the chitin cell wall of the fungus fusarium oxysporum f.sp cubense, inhibiting fungal proliferation.
 <!-- Add more about the biology of this part here