Difference between revisions of "Part:BBa K4137008:Design"

 
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===Design Notes===
 
===Design Notes===
−
he T7 promoter enables over production of mleR.
+
The T7 promoter enables over production of mleR.
 
RBS (AAGGAG) has been shown to be efficient in protein expression
 
RBS (AAGGAG) has been shown to be efficient in protein expression
 
MleR can be regulated by malate acid, which is presented around the roots. MleR will be further used as a signal for the production of antitoixen CcdA.
 
MleR can be regulated by malate acid, which is presented around the roots. MleR will be further used as a signal for the production of antitoixen CcdA.

Revision as of 02:12, 8 October 2022


mleR expressing construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 49
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The T7 promoter enables over production of mleR. RBS (AAGGAG) has been shown to be efficient in protein expression MleR can be regulated by malate acid, which is presented around the roots. MleR will be further used as a signal for the production of antitoixen CcdA. The Myc tag binds to mleR and allows the isolation of mleR for identification of individual concentrations



Source

https://www.snapgene.com/resources/plasmid-files/?set=pet_and_duet_vectors_(novagen)&plasmid=pET-28a(%2B) https://www.snapgene.com/resources/plasmid-files/?set=pet_and_duet_vectors_(novagen)&plasmid=pET-28a(%2B) https://journals.asm.org/doi/10.1128/jb.171.6.3108-3114.1989


References