Difference between revisions of "Part:BBa K4137011"

 
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<partinfo>BBa_K4137011 short</partinfo>
 
<partinfo>BBa_K4137011 short</partinfo>
  
−
Same as BBa_K4137011, but with 6xHis tag instead of Myc.
+
The above sequence encodes for the CcdB protein, a component of the CcdA-CcdB toxin-antitoxin system. CcdB binds with CcdA which inhibits CcdB from targeting DNA gyrase thus preventing DNA double-strand breaks. The 6xHis tag is used for protein purification and identification.
  
  

Revision as of 02:09, 8 October 2022


CcdB coding sequence + 6xHis

The above sequence encodes for the CcdB protein, a component of the CcdA-CcdB toxin-antitoxin system. CcdB binds with CcdA which inhibits CcdB from targeting DNA gyrase thus preventing DNA double-strand breaks. The 6xHis tag is used for protein purification and identification.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 234