Difference between revisions of "Part:BBa K4147002"
Line 3: Line 3:
 
<partinfo>BBa_K4147002 short</partinfo>
 
<partinfo>BBa_K4147002 short</partinfo>
  
−
This part contains the linear construct for (CBD)2-DrsB1 with an included LacI regulated promoter (1118 pb in total). Additionally, it contains an RBS under the part name BBa_B0032, as well as a PelB signal peptide and a 6X-His tag at the end of the dermaseptine CDS for later protein purification. Finally, it has a double terminator (BBa_B0015) to ensure correct termination. As a linear construct, it contains the RFC10 prefix and suffix, making the part compatible with other iGEM parts.  
+
This part contains the linear construct for (CBD)2-DrsB1 with an included LacI regulated promoter (1075 pb in total). Additionally, it contains an RBS under the part name BBa_B0032, as well as a PelB signal peptide and a 6X-His tag at the end of the dermaseptine CDS for later protein purification. Finally, it has a double terminator (BBa_B0015) to ensure correct termination. As a linear construct, it contains the RFC10 prefix and suffix, making the part compatible with other iGEM parts.  
 
Among a wide range of AMPs, polycationic dermaseptin peptides are produced by the skin glands of the frog <i>Phyllomedusa bicolor</i> [1]. It supplies both antibacterial and antifungal protection to a broad range of plant pathogens but shows no toxic effects on plant and mammalian cells [2]. This peptide of molecular weight of 22.1 kDa contains a tandem repeat of a chitin binding domain (CBD) as an improvement of dermaseptin-b1 gene (AKA MsrA2)(BBa_K2577001) which binds to chitin fungal cell wall, thereby increasing the lytic activity of the catalytic dom
 
Among a wide range of AMPs, polycationic dermaseptin peptides are produced by the skin glands of the frog <i>Phyllomedusa bicolor</i> [1]. It supplies both antibacterial and antifungal protection to a broad range of plant pathogens but shows no toxic effects on plant and mammalian cells [2]. This peptide of molecular weight of 22.1 kDa contains a tandem repeat of a chitin binding domain (CBD) as an improvement of dermaseptin-b1 gene (AKA MsrA2)(BBa_K2577001) which binds to chitin fungal cell wall, thereby increasing the lytic activity of the catalytic dom
  

Revision as of 22:46, 7 October 2022


Expression construct for (CBD)2-DrsB1: Dermaseptin AMP from Phyllomedusa bicolor

This part contains the linear construct for (CBD)2-DrsB1 with an included LacI regulated promoter (1075 pb in total). Additionally, it contains an RBS under the part name BBa_B0032, as well as a PelB signal peptide and a 6X-His tag at the end of the dermaseptine CDS for later protein purification. Finally, it has a double terminator (BBa_B0015) to ensure correct termination. As a linear construct, it contains the RFC10 prefix and suffix, making the part compatible with other iGEM parts. Among a wide range of AMPs, polycationic dermaseptin peptides are produced by the skin glands of the frog Phyllomedusa bicolor [1]. It supplies both antibacterial and antifungal protection to a broad range of plant pathogens but shows no toxic effects on plant and mammalian cells [2]. This peptide of molecular weight of 22.1 kDa contains a tandem repeat of a chitin binding domain (CBD) as an improvement of dermaseptin-b1 gene (AKA MsrA2)(BBa_K2577001) which binds to chitin fungal cell wall, thereby increasing the lytic activity of the catalytic dom

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 392
    Illegal AgeI site found at 671
  • 1000
    COMPATIBLE WITH RFC[1000]