Difference between revisions of "Part:BBa K4321004:Design"

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<partinfo>BBa_K4321004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4321004 SequenceAndFeatures</partinfo>
  
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===Design note===
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The Glycine at position 511 contains a GGC to a GGG silent mutation that destroys the NotI restriction site.
  
 
===Source===
 
===Source===

Revision as of 17:38, 7 October 2022


mScarlet


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 512
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design note

The Glycine at position 511 contains a GGC to a GGG silent mutation that destroys the NotI restriction site.

Source

This RFP was synthesized by Bindel et al (2017).The sequence was obtained from Snapgene and synthesized as a cassette (BBa_K4321007) by IDT.

References

Bindels, D., Haarbosch, L., van Weeren, L. et al. mScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nat Methods 14, 53–56 (2017). https://doi.org/10.1038/nmeth.4074

mScarlet . SnapGene. (n.d.). Retrieved October 4, 2022, from https://www.snapgene.com/resources/plasmid-files/?set=fluorescent_protein_genes_and_plasmids&plasmid=mScarlet