Difference between revisions of "Part:BBa K4180004"
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The enhanced GFP will be used as a control. It will be made into a composite part, pGal1, 10-eGFP-SBP (BBa_K4180008) as a control in the project
  
 
(enhanced green fluorescent protein) is used as control
 
(enhanced green fluorescent protein) is used as control

Revision as of 17:20, 7 October 2022


enhanced GFP (eGFP)

The enhanced GFP will be used as a control. It will be made into a composite part, pGal1, 10-eGFP-SBP (BBa_K4180008) as a control in the project (enhanced green fluorescent protein) is used as control The original GFP from the jellyfish Aequorea victoria was not bright enough to show the gene expression or the protein localization of several different organisms. Yang et al lab did several mutations on the GFP DNA sequence to enhance the brightness of GFP to make the detection much more sensitive

Sequence and Features

        BBa K4180004 eGFP
         PCR product

After Bacteria transformation, bacteria colonies were picking up directly to do eGFP PCR; colony#1 (lane2) has eGFP PCR product



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]