Difference between revisions of "Part:BBa K4165037"

 
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===Usage and Biology===
 
===Usage and Biology===
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Switch 17 is used to mediate the activity of HTRA1. Activating HTRA1 requires a conformational change in the linker, eliminating the attached inhibitor from the active site. The conformational rearrangement can be mediated through the affinity clamp for tau and beta-amyloid binding. These clamps are used for stabilizing the inhibitor away from the active site. These two domains (inhibitor and affinity clamp connected with linker1). Additionally, (H1A) binding peptide bound to the PDZ domain and connected to the affinity clamp on the other side with linker3
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4165037 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4165037 SequenceAndFeatures</partinfo>
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===Modeling===
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TRrosetta models this composite part with a score of 4 out of 6 according to our quality assessment code (you can find the python script file on the programming club page with further explanation of how you can optimize it to your needs).
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/17.png" style="margin-left:200px;" alt="" width="500" /></p>
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</html>
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                                Figure 1. The 3D structure of switch 17 modeled by TRrosetta
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Revision as of 16:55, 7 October 2022


HtrA1 Switch number 17

This composite part consists of T7 promoter (BBa_K3633015), lac operator (BBa_K4165062), pGS-21a RBS (BBa_K4165016), 6x His-tag (BBa_K4165020), WAP inhibitor (BBa_K4165008), GSGSG linker (BBa_K4165066), seed peptide (BBa_K4165012), GGSGGGGG linker (BBa_K4165019), seed peptide (BBa_K4165012), GSGSG linker (BBa_K4165066), H1A (BBa_K4165000) and T7 terminator (BBa_K731721).