Difference between revisions of "Part:BBa K4345007"

Revision as of 16:05, 7 October 2022

NarX

NarX is a part of NarX-NarL two component nitrate sensing system in Escherichia coli. They are responsible for the expression of anaerobic respiratory genes. NarX is a histidine kinases that independently detect the presence of nitrate an transmit the signal to NarL. After autophosphorylation and thus dimerization of NarX or NarQ, NarL is phosphorylated. This enables the activated NarL to bind DNA and induce expression of specific genes. Both NarX and narQ contain two conserved histidine residues that correspond to the autophosphorylation sites of other, homologous, sensor-transmitter proteins (Cavicchioli et al., 1995). Cheung & Hendrickson (2009) elucidated the structure of the histidine kinase in the apo- and holo-state to be a four-helix bundle.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 659
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 659
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 260
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 659
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 659
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4345007 short</partinfo>
-NarX-NarL two component system
+NarX is a part of NarX-NarL two component nitrate sensing system in Escherichia coli. They are responsible for the expression of anaerobic respiratory genes. NarX is a histidine kinases that independently detect the presence of nitrate an transmit the signal to NarL. After autophosphorylation and thus dimerization of NarX or NarQ, NarL is phosphorylated. This enables the activated NarL to bind DNA and induce expression of specific genes. Both NarX and narQ contain two conserved histidine residues that correspond to the autophosphorylation sites of other, homologous, sensor-transmitter proteins (Cavicchioli et al., 1995). Cheung & Hendrickson (2009) elucidated the structure of the histidine kinase in the apo- and holo-state to be a four-helix bundle.
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