Difference between revisions of "Part:BBa K4165032"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | < | + | Switch 12 is used to mediate the activity of HTRA1. Activating HTRA1 requires a conformational change in the linker, eliminating the attached inhibitor from the active site. The conformational rearrangement can be mediated through the affinity clamp for tau and beta-amyloid binding. These clamps are used for stabilizing the inhibitor away from the active site. These two domains (inhibitor and affinity clamp connected with linker1). Additionally, (H1A) binding peptide bound to the PDZ domain and connected to the affinity clamp on the other side with linker3 |
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| + | ===Modeling=== | ||
| + | TRrosetta models this composite part with a score of 6 out of 6 according to our quality assessment code (you can find the python script file on the programming club page with further explanation of how you can optimize it to your needs). | ||
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| + | <p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/12.png" style="margin-left:200px;" alt="" width="500" /></p> | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K4165032 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4165032 SequenceAndFeatures</partinfo> | ||
Revision as of 15:53, 7 October 2022
HtrA1 Switch number 12
This composite part consists of T7 promoter (BBa_K3633015), lac operator (BBa_K4165062), pGS-21a RBS (BBa_K4165016), 6x His-tag (BBa_K4165020), SPINK8 Inhibitor (BBa_K4165010), GS Linker (BBa_K4165017), seed peptide (BBa_K4165012), GS Linker (BBa_K4165019), seed peptide (BBa_K4165012), GS Linker (BBa_K4165017), H1A (BBa_K4165000) and T7 terminator (BBa_K731721).