Difference between revisions of "Part:BBa K4164004"

Revision as of 15:32, 7 October 2022

dimerization-dependent red fluorescent protein-A1(ddRFP-A1)

The dimerization-dependent red fluorescent protein-A1 (ddRFP-A1) is a variant of the dimeric Tomato, which is a monomer protein of the red fluorescent protein heterodimer, with a size of 26.4kDa.

ddRFP-A1 is an RFP variant with weak fluorescence derived from the directed evolution of dTomato gene. It has a chromogenic group, and can exhibit weak fluorescence in the monomeric state. ddRFPA1 can form a heterodimer with ddRFPB1, increasing the fluorescence intensity ten times more than the dissociation state. The utility of ddRFP-A1B1 has been demonstrated in three applications, including the detection of a protein-protein interaction in vitro, imaging of the reversible Ca2+-dependent association of calmodulin and imaging of caspase-3 activity during apoptosis.

In our project, we fused ddRFP-A1 and ddRFP-B1 to the C-terminus of FXR and RXR, respectively. Taking advantage of the characteristics that RXR can bind to the FXR as a heterodimer, using FXR as the receptor, and when the downstream ddRFP-A1 and ddRFP-B1 are correspondingly driven to dimerize and exhibit red fluorescence. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4164004 short</partinfo>
-ddRFPA1
+The dimerization-dependent red fluorescent protein-A1 (ddRFP-A1) is a variant of the dimeric Tomato, which is a monomer protein of the red fluorescent protein heterodimer, with a size of 26.4kDa.
+ddRFP-A1 is an RFP variant with weak fluorescence derived from the directed evolution of dTomato gene. It has a chromogenic group, and can exhibit weak fluorescence in the monomeric state. ddRFPA1 can form a heterodimer with ddRFPB1, increasing the fluorescence intensity ten times more than the dissociation state. The utility of ddRFP-A1B1 has been demonstrated in three applications, including the detection of a protein-protein interaction in vitro, imaging of the reversible Ca2+-dependent association of calmodulin and imaging of caspase-3 activity during apoptosis.
+In our project, we fused ddRFP-A1 and ddRFP-B1 to the C-terminus of FXR and RXR, respectively. Taking advantage of the characteristics that RXR can bind to the FXR as a heterodimer, using FXR as the receptor, and when the downstream ddRFP-A1 and ddRFP-B1 are correspondingly driven to dimerize and exhibit red fluorescence.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===