Difference between revisions of "Part:BBa K4268002:Experience"

Revision as of 15:16, 7 October 2022

The part was received, resuspended, and cloned into the level 0 Golden Gate vector, PSB1C00. The ligation was then transformed into DH5α cells. After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair.

Figure 1: Colony PCR of five colonies (clones) suspected of coating the insert capsid assembly protein insert. The insert length is 769bp and the predicted size of the PCR product when using VF/VR primers is 1739 bp.

The gel indicates that all five colonies are likely to contain the correct insert and thus, the _ was successfully cloned into a Level O Golden Gate Assembly basic part.

Applications of BBa_K4268002

User Reviews

UNIQ489d2d27409afcec-partinfo-00000000-QINU UNIQ489d2d27409afcec-partinfo-00000001-QINU

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 __NOTOC__
-This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
+The part was received, resuspended, and cloned into the level 0 Golden Gate vector, PSB1C00. The ligation was then transformed into DH5&alpha; cells.  After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair.
-how you used this part and how it worked out.
+[[File: T-suny-oneonta-t7-capsid protein colony PCR figure.png|500px|thumb|center|Figure 1: Colony PCR of five colonies (clones) suspected of coating the insert capsid assembly protein insert. The insert length is 769bp and the predicted size of the PCR product when using VF/VR primers is 1739 bp.]]
+The gel indicates that all five colonies are likely to contain the correct insert and thus, the _ was successfully cloned into a Level O Golden Gate Assembly basic part.
 ===Applications of BBa_K4268002===