Difference between revisions of "Part:BBa K4165210"

Revision as of 12:20, 7 October 2022

Coh2 linked with tau binding peptide (WINK) & GSGS linker

Fusion protein consists of Tau binding peptide (WINK) for tau targeting and Coh2 protein [BBa_K4165003] that bind to Docs [BBa_K3396000] to form the protac system.

Usage and Biology

Protac system is known to be a promising one for targeting and signaling many diseases and biological application so according to our project aim , we introduced this fusion protein with different linker length and tau binding peptide to be a target for degradation of Tau aggregations . Furthermore, that can be validated by wet-lab results by in vitro or in vivo testing.

3D Modeling and Ranking

Fused protein consists of WINK tau binding peptide (BBa_K4165146) with linker (GS)2 (BBa_K4165065) to link between the binding peptide and Coh2 (BBa_K4165003). this model was designed by Modeller tool with QA 5 out of 6 according to our parameters in QA code: C-beta deviations , clash score, Molprobity, Ramachandran favored, Ramachandran outliers, Q mean 4, and Q mean 6. This protein was excluded due to its decrease in the overall protein stability as compared to other models. C-beta deviations = 2 , clash score = 39.86 , Molprobity = 2.08 , Ramachandran favored = 98.67 , Ramachandran outliers = 0 , Q mean 4 = 0.1 , Q mean 6 = -0.01

                      Figure 1.: Predicted 3D structure of CoH2-(GS)2-WINK protein.

Sequence and Features