Difference between revisions of "Part:BBa K4231016"

Revision as of 11:27, 7 October 2022

Y.lipolytica TEF1 promoter with a regulatory sequence

The length of A1 is 400 bp, the length of R1 is 200 bp, and the length of A3 is 338 bp. The three parts are assembled together to form the UAS sequence of the inducible TEF promoter. Among them, R1 sequence plays the role of oleic acid induction, A1 and A3 sequences play the role of enhancing transcription activity. Considering the limited length and capacity of upstream sequence, we choose a single-repeat R1 sequence to construct UAS sequence. The promoter carrying this UAS can be strongly induced by oleic acid, and when the carbon source is glucose, the transcription activity is almost zero. This mixed promoter induced by oleic acid is nearly three times stronger than the native POX2 promoter and is a powerful tool for regulating and enhancing gene expression.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 797
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 797
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 797
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4231016 short</partinfo>
-Translation elongation factor-1&#945; (TEF1) promoter from the Yarrowia lipolytica with a regulatory sequence called Activating1-Regulatory1-Activating3(A1-R1-A3) can be strongly induced by oleic acid.
+The length of A1 is 400 bp, the length of R1 is 200 bp, and the length of A3 is 338 bp. The three parts are assembled together to form the UAS sequence of the inducible TEF promoter. Among them, R1 sequence plays the role of oleic acid induction, A1 and A3 sequences play the role of enhancing transcription activity. Considering the limited length and capacity of upstream sequence, we choose a single-repeat R1 sequence to construct UAS sequence. The promoter carrying this UAS can be strongly induced by oleic acid, and when the carbon source is glucose, the transcription activity is almost zero. This mixed promoter induced by oleic acid is nearly three times stronger than the native POX2 promoter and is a powerful tool for regulating and enhancing gene expression.
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