Difference between revisions of "Part:BBa K4121019:Design"

 
 
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===Design Notes===
 
===Design Notes===
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Before using this part, give priority to whether it has the enzyme cleavage site required, if so, consider site-directed mutagenesis.
+
Before using this part, please check to whether the operated sequences contain any enzyme site required for the construction. If so, please consider to change it using site-directed mutagenesis.
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+
  
  

Latest revision as of 11:16, 7 October 2022


The HMG-CoA reductase gene HMG1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1311
    Illegal PstI site found at 5
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 5
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 685
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1311
    Illegal PstI site found at 5
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1311
    Illegal PstI site found at 5
    Illegal NgoMIV site found at 1456
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Before using this part, please check to whether the operated sequences contain any enzyme site required for the construction. If so, please consider to change it using site-directed mutagenesis.


Source

National Center for Biotechnology Information (NCBI)

References