Difference between revisions of "Part:BBa I742142"
Line 3: Line 3:
  
 
This is the coding sequence of sam8, encoding tyrosine ammonia lyase, from the bacterium ''Saccharothrix espanaensis'' DSM 4429. The enzyme converts tyrosine to p-coumaric acid. Accession: ABC88666. Reference: Berner, M., Krug, D., Bihlmaier, C., Vente, A., Muller, R. and Bechthold, A. 'Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete ''Saccharothrix espanaensis''' J. Bacteriol. 188 (7), 2666-2673 (2006)
 
This is the coding sequence of sam8, encoding tyrosine ammonia lyase, from the bacterium ''Saccharothrix espanaensis'' DSM 4429. The enzyme converts tyrosine to p-coumaric acid. Accession: ABC88666. Reference: Berner, M., Krug, D., Bihlmaier, C., Vente, A., Muller, R. and Bechthold, A. 'Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete ''Saccharothrix espanaensis''' J. Bacteriol. 188 (7), 2666-2673 (2006)
−
 
+
<p>
−
iGEM2022-UCAS-China-Contribution
+
<b>iGEM2022-UCAS-China Contribution</b><br data-filtered="filtered">
 
We used <i>sam8</i> in one of our plasmids for the production of ferulic acid from tyrosine: <i>sam8-comt-sam5</i>/pet-28a for vanillin synthesis BBa_K4256012.
 
We used <i>sam8</i> in one of our plasmids for the production of ferulic acid from tyrosine: <i>sam8-comt-sam5</i>/pet-28a for vanillin synthesis BBa_K4256012.
 +
</p>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 09:42, 7 October 2022

sam8 (tyrosine-ammonia lyase) coding sequence

This is the coding sequence of sam8, encoding tyrosine ammonia lyase, from the bacterium Saccharothrix espanaensis DSM 4429. The enzyme converts tyrosine to p-coumaric acid. Accession: ABC88666. Reference: Berner, M., Krug, D., Bihlmaier, C., Vente, A., Muller, R. and Bechthold, A. 'Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix espanaensis' J. Bacteriol. 188 (7), 2666-2673 (2006)

iGEM2022-UCAS-China Contribution
We used sam8 in one of our plasmids for the production of ferulic acid from tyrosine: sam8-comt-sam5/pet-28a for vanillin synthesis BBa_K4256012.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 794
    Illegal BamHI site found at 1196
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 537
    Illegal AgeI site found at 484
    Illegal AgeI site found at 653
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 88
    Illegal BsaI site found at 354