Difference between revisions of "Part:BBa K4385018:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
It is mainly composed of constitutive promoter(BBa_J23100) , the anchoring protein OmpC-ZBP(BBa_K4385003, BBa_K4385004) and Heavy metal detoxification protein( BBa_K4385010).
+
It is mainly composed of pbr promoter (BBa_K4385009) , the anchoring protein Lpp-OmpA(BBa_K4385001, BBa_K4385002), the lead ion adsorbed protein PbrR(BBa_K43885000)and heavy metal detoxification protein( BBa_K4385010).
  
  

Latest revision as of 09:38, 7 October 2022


Lead-inducible surface display


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1627
    Illegal PstI site found at 462
    Illegal PstI site found at 1569
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1809
    Illegal NheI site found at 1832
    Illegal SpeI site found at 1627
    Illegal PstI site found at 462
    Illegal PstI site found at 1569
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1944
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1627
    Illegal PstI site found at 462
    Illegal PstI site found at 1569
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1627
    Illegal PstI site found at 462
    Illegal PstI site found at 1569
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 253
    Illegal BsaI site found at 1360


Design Notes

It is mainly composed of pbr promoter (BBa_K4385009) , the anchoring protein Lpp-OmpA(BBa_K4385001, BBa_K4385002), the lead ion adsorbed protein PbrR(BBa_K43885000)and heavy metal detoxification protein( BBa_K4385010).


Source

The basic parts that make up the composite part are all derived from E. coli genomic DNA.

References