Difference between revisions of "Part:BBa K4385018"
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<partinfo>BBa_K4385018 short</partinfo> | <partinfo>BBa_K4385018 short</partinfo> | ||
| − | .. | + | The part mainly consists of the lead-inducible promoter pbr , the anchoring protein Lpp-OmpA and the heavy metal detoxification protein metallothionein. When the lead ion concentration in the environment exceeds a certain concentration, the pbr promoter initiates transcription of downstream OmpC-CBP, witch is used as an anchoring protein to anchor PbrR on the surface of E. coli for lead ion adsorption. |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 09:27, 7 October 2022
Lead-inducible surface display
The part mainly consists of the lead-inducible promoter pbr , the anchoring protein Lpp-OmpA and the heavy metal detoxification protein metallothionein. When the lead ion concentration in the environment exceeds a certain concentration, the pbr promoter initiates transcription of downstream OmpC-CBP, witch is used as an anchoring protein to anchor PbrR on the surface of E. coli for lead ion adsorption.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 1627
Illegal PstI site found at 462
Illegal PstI site found at 1569 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1809
Illegal NheI site found at 1832
Illegal SpeI site found at 1627
Illegal PstI site found at 462
Illegal PstI site found at 1569 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1944
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 1627
Illegal PstI site found at 462
Illegal PstI site found at 1569 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 1627
Illegal PstI site found at 462
Illegal PstI site found at 1569 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 253
Illegal BsaI site found at 1360