Difference between revisions of "Part:BBa K4140002"
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We use this part to increase the expression of TyrR by increasing the rate of ribosomal recruitment and the ability to start and influences the precision and effectiveness with which the mRNA translation process codon. as shown in figure 1. | We use this part to increase the expression of TyrR by increasing the rate of ribosomal recruitment and the ability to start and influences the precision and effectiveness with which the mRNA translation process codon. as shown in figure 1. | ||
| − | [[File:tyrr.png|thumb|Right|Figure 1. (shows usage of TyrR RBS in our circuit design) ]] | + | [[File:tyrr.png|thumb|Right|Figure 1. (shows the usage of TyrR RBS in our circuit design) ]] |
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Revision as of 09:25, 7 October 2022
TyrR RBS
Part Description
Sequence upstream of the start codon is crucial for the recruitment of a ribosome during the initiation of translation, which influences the rate and efficiency at which a certain Open Reading Frame (ORF) is translated by the following mechanisms:
-the rate of ribosomal recruitment to RBS
-the speed at which a recruited ribosome is able to start translation (i.e. the translation initiation efficiency)
-The ideal spacing of the RBS from the start codon
Usage
We use this part to increase the expression of TyrR by increasing the rate of ribosomal recruitment and the ability to start and influences the precision and effectiveness with which the mRNA translation process codon. as shown in figure 1.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]