Difference between revisions of "Part:BBa K4165208"

 
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Fusion protein consists of Tau binding peptide (M4W39) for tau targeting and Coh2 protein [BBa_K4165003] that bind to Docs [BBa_K3396000] to form the protac system with linker GSGS [BBa_K4165065]
 
Fusion protein consists of Tau binding peptide (M4W39) for tau targeting and Coh2 protein [BBa_K4165003] that bind to Docs [BBa_K3396000] to form the protac system with linker GSGS [BBa_K4165065]
  
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===Usage and Biology===
 
===Usage and Biology===
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Protac system is known to be a promising one for targeting and signaling many diseases and biological application so according to our project aim . we introduced this fusion protein with different linker length and tau binding peptide to be a target for degradation of Tau aggregations . Furthermore, that can be validated by wet-lab results by in vitro or in vivo testing.
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===3D Modeling and Ranking===
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This fused protein was modeled using Robetta software and after running our quality assessment code it got a score of 4 out of 6 according to the parameters of the QA code: C-beta deviations, clash score, Molprobity, Ramachandran favored, Ramachandran outliers, Q mean 4, and Q mean 6.
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This protein was excluded due to its low QA score as compared to other models.
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/g2s.png" style="margin-left:200px;" alt="" width="500" /></p>
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                                          Figure 1. Predicted 3D structure of CoH2-(GSGS)-M4W39 protein.
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Revision as of 08:59, 7 October 2022


Coh2 linked with tau binding peptide (M4W39) and GSGS linker

Fusion protein consists of Tau binding peptide (M4W39) for tau targeting and Coh2 protein [BBa_K4165003] that bind to Docs [BBa_K3396000] to form the protac system with linker GSGS [BBa_K4165065]


Usage and Biology

Protac system is known to be a promising one for targeting and signaling many diseases and biological application so according to our project aim . we introduced this fusion protein with different linker length and tau binding peptide to be a target for degradation of Tau aggregations . Furthermore, that can be validated by wet-lab results by in vitro or in vivo testing.

3D Modeling and Ranking

This fused protein was modeled using Robetta software and after running our quality assessment code it got a score of 4 out of 6 according to the parameters of the QA code: C-beta deviations, clash score, Molprobity, Ramachandran favored, Ramachandran outliers, Q mean 4, and Q mean 6. This protein was excluded due to its low QA score as compared to other models. 

                                         Figure 1. Predicted 3D structure of CoH2-(GSGS)-M4W39 protein.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]