Difference between revisions of "Part:BBa K4375003"

Revision as of 08:06, 7 October 2022

Modified Constitutive Promoter (J23101)

J23101* is part of the J23, Anderson constitutive promoter family, it is the modified version J23101 Anderson promoter (BBa_J23101). It is weaker than the original promoter.

Usage and Biology

This promoter was used to regulate BLADE protein transcription in ELTE 2022 group’s project. It was important that this promoter by the literature is weaker than J23101 Anderson promoter, because in this case the production of the protein means less stress for the bacteria.

This promoter is shorter than the J23101. The nucleotid with the red frame is missing from it. This change leads to a weaker promoter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 29
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Reference

https://www.nature.com/articles/s41589-021-00787-6.pdf

@@ Line 5: / Line 5: @@
 J23101* is part of the J23, Anderson constitutive promoter family, it is the modified version J23101 Anderson promoter (BBa_J23101). It is weaker than the original promoter.
+==Usage and Biology==
+This promoter was used to regulate BLADE protein transcription in ELTE 2022 group’s project. It was important that this promoter by the literature is weaker than J23101 Anderson promoter, because in this case the production of the protein means less stress for the bacteria.
+This promoter is shorter than the J23101. The nucleotid with the red frame is missing from it. This change leads to a weaker promoter.
-<!-- Add more about the biology of this part here
-===Usage and Biology===
 <!-- -->
@@ Line 14: / Line 18: @@
 <partinfo>BBa_K4375003 SequenceAndFeatures</partinfo>
+==Reference==
+https://www.nature.com/articles/s41589-021-00787-6.pdf
 <!-- Uncomment this to enable Functional Parameter display