Difference between revisions of "Part:BBa K4162028"
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<partinfo>BBa_K4162028 short</partinfo>
 
<partinfo>BBa_K4162028 short</partinfo>
  
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Gene ybbO tagged with tdMCP can be combined with the stem ring structure of MS2, so that retinol dehydrogenase expressed by gene ybbO can be enriched in the area of TEARS. This part can be used to assemble polycistrons in Escherichia coli.
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===Introduction===
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[[File:T--Fudan--logo-Rester-rectangle.jpg|100px|right|2021 Fudan]]
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Gene ''ybbO'' tagged with tdMCP can be combined with the stem ring structure of MS2, so that retinol dehydrogenase expressed by gene ''ybbO'' can be enriched in the area of TEARS. This part can be used to assemble polycistrons in ''E. coli''.
  
 
<!-- Add more about the biology of this part here
 
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Revision as of 07:48, 7 October 2022


ribozyme + T7_RBS + ybbO-tdMCP-EGFP

Introduction

2021 Fudan

Gene ybbO tagged with tdMCP can be combined with the stem ring structure of MS2, so that retinol dehydrogenase expressed by gene ybbO can be enriched in the area of TEARS. This part can be used to assemble polycistrons in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1612
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1612
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1612
    Illegal AgeI site found at 109
    Illegal AgeI site found at 702
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1192
    Illegal BsaI site found at 1546