Difference between revisions of "Part:BBa K4192110"
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===Characterization=== | ===Characterization=== | ||
− | <p>This part has been connected to the downstream of Plac and transferred into <i>E. coli</i> and <i>Pseudomonas fluorescens</i> 2P24 for testing. To get more information about the resut, please see https://parts.igem.org/Part:BBa_K4192114. | + | <p>This part has been connected to the downstream of Plac and transferred into <i>E. coli</i> and <i>Pseudomonas fluorescens</i> 2P24 for testing. To get more information about the resut, please see https://parts.igem.org/Part:BBa_K4192114.</p> |
− | The results showed that it was difficult to express in <i>E. coli</i>, but it had a good expression effect in <i>Pseudomonas fluorescens</i> 2P24, which means that the two genes can be co-expressed to improve the biofilm production of <i>Pseudomonas fluorescens</i>. | + | <p>The results showed that it was difficult to express in <i>E. coli</i>, but it had a good expression effect in <i>Pseudomonas fluorescens</i> 2P24, which means that the two genes can be co-expressed to improve the biofilm production of <i>Pseudomonas fluorescens</i>.</p> |
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Revision as of 07:27, 7 October 2022
cdg-gacA
RBS-cdg-RBS-gacA is composed of cdg gene (BBa_K4192010), gacA gene (BBa_4192011) and strong RBS sequence of Pseudomonas fluorescens. (BBa_K4192000). cdg and gacA encode regulatory factors DGC and GacA in Pseudomonas fluorescens, which can ncrease the expression of extracellular polysaccharide (EPS) and then increase the production of biofilm.
Characterization
This part has been connected to the downstream of Plac and transferred into E. coli and Pseudomonas fluorescens 2P24 for testing. To get more information about the resut, please see https://parts.igem.org/Part:BBa_K4192114.
The results showed that it was difficult to express in E. coli, but it had a good expression effect in Pseudomonas fluorescens 2P24, which means that the two genes can be co-expressed to improve the biofilm production of Pseudomonas fluorescens.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 268
Illegal BamHI site found at 259
Illegal XhoI site found at 357
Illegal XhoI site found at 801 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 192
Illegal NgoMIV site found at 1289
Illegal AgeI site found at 759 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 210
Illegal BsaI.rc site found at 777