Difference between revisions of "Part:BBa K4192110"
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RBS-cdg-RBS-gacA is composed of <i>cdg</i> gene (BBa_K4192010), <i>gacA</i> gene (BBa_4192011) and strong RBS sequence of <i>Pseudomonas fluorescens</i>. (BBa_K4192000).
 
RBS-cdg-RBS-gacA is composed of <i>cdg</i> gene (BBa_K4192010), <i>gacA</i> gene (BBa_4192011) and strong RBS sequence of <i>Pseudomonas fluorescens</i>. (BBa_K4192000).
 
<i>cdg</i> and <i>gacA</i> encode regulatory factors DGC and GacA in <i>Pseudomonas fluorescens</i>, which can ncrease the expression of extracellular polysaccharide (EPS) and then increase the production of biofilm.
 
<i>cdg</i> and <i>gacA</i> encode regulatory factors DGC and GacA in <i>Pseudomonas fluorescens</i>, which can ncrease the expression of extracellular polysaccharide (EPS) and then increase the production of biofilm.
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This part has been connected to the downstream of Plac and transferred into <i>E. coli</i> and <i>Pseudomonas fluorescens</i> 2P24 for testing. The data are as follows:
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The results showed that it was difficult to express in <i>E. coli</i>, but it had a good expression effect in <i>Pseudomonas fluorescens</i> 2P24.
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===Characterization===
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<p>This part has been connected to the downstream of Plac and transferred into <i>E. coli</i> and <i>Pseudomonas fluorescens</i> 2P24 for testing. To get more information about the resut, please see https://parts.igem.org/Part:BBa_K4192114.
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The results showed that it was difficult to express in <i>E. coli</i>, but it had a good expression effect in <i>Pseudomonas fluorescens</i> 2P24, which means that the two genes can be co-expressed to improve the biofilm production of <i>Pseudomonas fluorescens</i>.
  
 
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Revision as of 07:27, 7 October 2022


cdg-gacA

RBS-cdg-RBS-gacA is composed of cdg gene (BBa_K4192010), gacA gene (BBa_4192011) and strong RBS sequence of Pseudomonas fluorescens. (BBa_K4192000). cdg and gacA encode regulatory factors DGC and GacA in Pseudomonas fluorescens, which can ncrease the expression of extracellular polysaccharide (EPS) and then increase the production of biofilm.

Characterization

This part has been connected to the downstream of Plac and transferred into E. coli and Pseudomonas fluorescens 2P24 for testing. To get more information about the resut, please see https://parts.igem.org/Part:BBa_K4192114. The results showed that it was difficult to express in E. coli, but it had a good expression effect in Pseudomonas fluorescens 2P24, which means that the two genes can be co-expressed to improve the biofilm production of Pseudomonas fluorescens. Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 268
    Illegal BamHI site found at 259
    Illegal XhoI site found at 357
    Illegal XhoI site found at 801
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 192
    Illegal NgoMIV site found at 1289
    Illegal AgeI site found at 759
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 210
    Illegal BsaI.rc site found at 777