Difference between revisions of "Part:BBa K274120"

Revision as of 06:16, 19 September 2009

CrtEBI under pBad promoter

This Biobrick is created by putting enzyme cassette CrtEBI (with individual rbs) of Part BBa_K274100 under arabinose-induced promoter I0500.

Enzyme cassette CrtEBI (with individual rbs) of Part BBa_K274100 converts colourless farnesyl pyrophosphate to red lycopene (via intermediates geranylgeranyl pyroiphosphate and phytoene).

Amount of lycopene produced can be measured by photospectrometer with absorbance at 475nm (lycopene extraction using acetone).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1205
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1144
Illegal BamHI site found at 3192
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 2728
Illegal NgoMIV site found at 2858
Illegal AgeI site found at 979
Illegal AgeI site found at 1943
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 961

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K274120 short</partinfo>
@@ Line 5: / Line 4: @@
 This Biobrick is created by putting enzyme cassette CrtEBI (with individual rbs) of Part BBa_K274100 under arabinose-induced promoter I0500.
-Enzyme cassette CrtEBI (with individual rbs) of Part BBa_K274110 converts colourless farnesyl pyrophosphate to red lycopene (via intermediates geranylgeranyl pyroiphosphate and phytoene).
+Enzyme cassette CrtEBI (with individual rbs) of Part BBa_K274100 converts colourless farnesyl pyrophosphate to red lycopene (via intermediates geranylgeranyl pyroiphosphate and phytoene).
 Amount of lycopene produced can be measured by photospectrometer with absorbance at 475nm (lycopene extraction using acetone).