Difference between revisions of "Part:BBa K4417000"
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<h1>Description</h1>
 
<h1>Description</h1>
 
Shuttle vecor for Bacillus subtilis. Mutated pCT5-bac2.0 by removing three BsaI sites to make it TypeIIS compatible.
 
Shuttle vecor for Bacillus subtilis. Mutated pCT5-bac2.0 by removing three BsaI sites to make it TypeIIS compatible.
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<h1>Usage and Biology</h1>
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<h1>References</h1>
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<p>1.  Development of a synthetic cumate-inducible gene expression system for Bacillus. Seo SO, Schmidt-Dannert C. Appl Microbiol Biotechnol. 2018 Nov 3. pii: 10.1007/s00253-018-9485-4. doi: 10.1007/s00253-018-9485-4. 10.1007/s00253-018-9485-4 PubMed 30392122 </p>
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<p>2. Addgene plasmid # 119872 ; http://n2t.net/addgene:119872 ; RRID:Addgene_119872 </p>
  
 
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Revision as of 23:40, 6 October 2022


pCT5c Type IIS Compatible Plasmid

Description

Shuttle vecor for Bacillus subtilis. Mutated pCT5-bac2.0 by removing three BsaI sites to make it TypeIIS compatible.

Usage and Biology

References

1. Development of a synthetic cumate-inducible gene expression system for Bacillus. Seo SO, Schmidt-Dannert C. Appl Microbiol Biotechnol. 2018 Nov 3. pii: 10.1007/s00253-018-9485-4. doi: 10.1007/s00253-018-9485-4. 10.1007/s00253-018-9485-4 PubMed 30392122

2. Addgene plasmid # 119872 ; http://n2t.net/addgene:119872 ; RRID:Addgene_119872

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3926
    Illegal EcoRI site found at 6982
    Illegal XbaI site found at 7437
    Illegal SpeI site found at 1
    Illegal PstI site found at 3174
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3926
    Illegal EcoRI site found at 6982
    Illegal NheI site found at 7213
    Illegal SpeI site found at 1
    Illegal PstI site found at 3174
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3926
    Illegal EcoRI site found at 6982
    Illegal BamHI site found at 907
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3926
    Illegal EcoRI site found at 6982
    Illegal XbaI site found at 7437
    Illegal SpeI site found at 1
    Illegal PstI site found at 3174
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3926
    Illegal EcoRI site found at 6982
    Illegal XbaI site found at 7437
    Illegal SpeI site found at 1
    Illegal PstI site found at 3174
    Illegal NgoMIV site found at 1937
  • 1000
    COMPATIBLE WITH RFC[1000]