Difference between revisions of "Part:BBa K4368006:Design"

 
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===Design Notes===
 
===Design Notes===
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No special considerations.
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===Source===
 
===Source===
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This part was derived from the reporter device used by Elowitz's repressilator Elowitz. Elowitz obtained the GFP tagged sequence (gfpmut3*) published by Andersen and coworkers Andersen to build the reporter device. Andersen constructed those GFP variants based on a gfpmut3b containing plasmid that was a gift from R. H. Valdivia Valdivia. The PCR method used in construction by Andersen changed aa residue 2 from serine to arginine with no detectable phenotypic effect in their experiments.
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===References===
 
===References===

Revision as of 23:03, 6 October 2022


pcstA + rbs + GFP + terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 804


Design Notes

No special considerations.


Source

This part was derived from the reporter device used by Elowitz's repressilator Elowitz. Elowitz obtained the GFP tagged sequence (gfpmut3*) published by Andersen and coworkers Andersen to build the reporter device. Andersen constructed those GFP variants based on a gfpmut3b containing plasmid that was a gift from R. H. Valdivia Valdivia. The PCR method used in construction by Andersen changed aa residue 2 from serine to arginine with no detectable phenotypic effect in their experiments.

References