Difference between revisions of "Part:BBa K4165108"

Revision as of 20:45, 6 October 2022

HTRA Binding Peptide 14

part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein

Usage and Biology

decameric peptide, Binds specifically to PDZ domain of HTRA1 Protein with G of 3.8 ± 0.2 kcal/mol.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Functional Parameters

Got score of 5 out of 6 in our Quality assessment code after modeling by AlphaFold2.0

cbeta_deviations = 0, clashscore = 0, molprobity = 1.62, ramachandran_favored = 100, ramachandran_outliers = 0, Qmean_4 = -5.843485, Qmean6 = -4.746417

Modeling

                 Figure 1.: 3D Model of H1C peptide Modelled by AlphaFold2.0 displayed in Pyomol

@@ Line 5: / Line 5: @@
 part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+decameric peptide, Binds specifically to PDZ domain of HTRA1 Protein with G of 3.8 ± 0.2 kcal/mol.
 <!-- -->
@@ Line 14: / Line 12: @@
 <partinfo>BBa_K4165108 SequenceAndFeatures</partinfo>
-<!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
+Got score of 5 out of 6 in our Quality assessment code after modeling by AlphaFold2.0
+cbeta_deviations = 0, clashscore = 0, molprobity = 1.62, ramachandran_favored = 100, ramachandran_outliers = 0, Qmean_4 = -5.843485, Qmean6 = -4.746417
+===Modeling===
+<html>
+<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/htra1-bp/h1n.jpg" style="margin-left:200px;" alt="" width="500" /></p>
+</html>
+                  Figure 1.: 3D Model of H1C peptide Modelled by AlphaFold2.0 displayed in Pyomol
+===Docking===
 <partinfo>BBa_K4165108 parameters</partinfo>
 <!-- -->