Difference between revisions of "Part:BBa K4165097"

Revision as of 20:10, 6 October 2022

HTRA Binding Peptide 3

part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein

Usage and Biology

Synthetic peptide acquired by Phage display, Binds specifically to PDZ domain of HTRA1 and HTRA3 Proteins with G of –8.2 and IC50 of 2.5 ± 0.4

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Functional Parameters

Got score of 5 out of 6 in our Quality assessment code after modeling by AlphaFold2.0

cbeta_deviations = 0, clashscore = 129.77, molprobity = 4.76, ramachandran_favored = 25, ramachandran_outliers = 75, Qmean_4 = -6.45183, Qmean6 = -5.513969

Modeling

                 Figure 1.: 3D Model of H1C peptide Modelled by AlphaFold2.0 displayed in Pyomol

@@ Line 1: / Line 1: @@
 __NOTOC__
-<partinfo>BBa_K4165096 short</partinfo>
+<partinfo>BBa_K4165097 short</partinfo>
 part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein
@@ Line 11: / Line 11: @@
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>
-<partinfo>BBa_K4165096 SequenceAndFeatures</partinfo>
+<partinfo>BBa_K4165097 SequenceAndFeatures</partinfo>
 ===Functional Parameters===
 Got score of 5 out of 6 in our Quality assessment code after modeling by AlphaFold2.0
 cbeta_deviations = 0, clashscore = 129.77, molprobity = 4.76, ramachandran_favored = 25, ramachandran_outliers = 75, Qmean_4 = -6.45183, Qmean6 = -5.513969
@@ Line 27: / Line 28: @@
-<partinfo>BBa_K4165096 parameters</partinfo>
+<partinfo>BBa_K4165097 parameters</partinfo>
 <!-- -->