Difference between revisions of "Part:BBa K4245003:Design"

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===References===
 
===References===
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Autour, A., Westhof, E., & Ryckelynck, M. (2016). iSpinach: A fluorogenic RNA aptamer optimized for in vitro applications. Nucleic Acids Research, 44(6), 2491–2500. https://doi.org/10.1093/nar/gkw083
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Neubacher, S., & Hennig, S. (2018). RNA structure and cellular applications of fluorescent light-up Aptamers. Angewandte Chemie International Edition, 58(5), 1266–1279. https://doi.org/10.1002/anie.201806482
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Paige, J. S., Wu, K. Y., & Jaffrey, S. R. (2011). RNA mimics of green fluorescent protein. Science, 333(6042), 642–646. https://doi.org/10.1126/science.1207339

Revision as of 20:09, 6 October 2022


iSpinach-D5-G30-A32 aptamer with LacI repression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 366
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The iSpinach aptamer was originally generated by the researchers at the University of Strasbourg using the SELEX (systematic evolution of ligands by exponential enrichment) process (Millan et. al, 2017).

Source

The part was acquired as synthesized sequences from IDT and Twist Bioscience.

References

Autour, A., Westhof, E., & Ryckelynck, M. (2016). iSpinach: A fluorogenic RNA aptamer optimized for in vitro applications. Nucleic Acids Research, 44(6), 2491–2500. https://doi.org/10.1093/nar/gkw083

Neubacher, S., & Hennig, S. (2018). RNA structure and cellular applications of fluorescent light-up Aptamers. Angewandte Chemie International Edition, 58(5), 1266–1279. https://doi.org/10.1002/anie.201806482

Paige, J. S., Wu, K. Y., & Jaffrey, S. R. (2011). RNA mimics of green fluorescent protein. Science, 333(6042), 642–646. https://doi.org/10.1126/science.1207339