Difference between revisions of "Part:BBa K4239003"

Revision as of 19:20, 6 October 2022

Enhanced luciferase substrate forming units fiatluxA

fiatluxA is made to be used with fiatluxB. It codes for a subpart of the luciferase protein. With the subpart coding from fiatluxD, they form the luciferase protein.

Luciferase has as substrats FMNH2, O2 and Fatty aldehydes, and produces H20, Fatty Acids and FMN and emits luminescence.

The systeme fiatluxA/fiatluxB is made to be used with fiatluxC, fiatluxD and fiatluxE, gathered in the fiatluxCDABE operon.

Fiatlux genes come from ilux genes (C, D, A, B, E). They were modified to remove every Igem restriction site (EcoR1, Xba1, Spe1 and Pst1) included in genes. They were also adapted to include the biobrick format.

The ilux operon was born from a mutated natural luminescence operon present in the bacteria P.luminescens: the lux operon. These mutations were error-prone PCR induced according to Gregor et al.’s study in 2018 (Gregor et al. 2018). The aim was to create a system of genes that produced more light.

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 504
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 1023

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 <partinfo>BBa_K4239003 short</partinfo>
-aze
+fiatluxA is made to be used with fiatluxB. It codes for a subpart of the luciferase protein. With the subpart coding from fiatluxD, they form the luciferase protein.
+Luciferase has as substrats FMNH2, O2 and Fatty aldehydes, and produces H20, Fatty Acids and FMN and emits luminescence.
+The systeme fiatluxA/fiatluxB is made to be used with fiatluxC, fiatluxD and fiatluxE, gathered in the fiatluxCDABE operon.
+Fiatlux genes come from ilux genes (C, D, A, B, E). They were modified to remove every Igem restriction site (EcoR1, Xba1, Spe1 and Pst1) included in genes. They were also adapted to include the biobrick format.
+The ilux operon was born from a mutated natural luminescence operon present in the bacteria P.luminescens: the lux operon. These mutations were error-prone PCR induced according to Gregor et al.’s study in 2018 (Gregor et al. 2018). The aim was to create a system of genes that produced more light.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===