Difference between revisions of "Part:BBa K4245000:Design"

(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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+
The iSpinach aptamer was originally generated by the researchers at the University of Strasbourg using the SELEX (systematic evolution of ligands by exponential enrichment) process (https://academic.oup.com/nar/article/44/6/2491/2499459).
 
===Source===
 
===Source===
−
 
+
The part was acquired as synthesized sequences from IDT and Twist Bioscience.
 
===References===
 
===References===
 +
Fernandez-Millan, P., Autour, A., Ennifar, E., Westhof, E., & Ryckelynck, M. (2017). Crystal structure and fluorescence properties of the iSpinach aptamer in complex with DFHBI. RNA, 23(12), 1788–1795. https://doi.org/10.1261/rna.063008.117
 +
 +
Neubacher, S., & Hennig, S. (2018). RNA structure and cellular applications of fluorescent light-up Aptamers. Angewandte Chemie International Edition, 58(5), 1266–1279. https://doi.org/10.1002/anie.201806482
 +
 +
Paige, J. S., Wu, K. Y., & Jaffrey, S. R. (2011). RNA mimics of green fluorescent protein. Science, 333(6042), 642–646. https://doi.org/10.1126/science.1207339

Revision as of 18:47, 6 October 2022


iSpinach-D5-G30-A32 Aptamer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The iSpinach aptamer was originally generated by the researchers at the University of Strasbourg using the SELEX (systematic evolution of ligands by exponential enrichment) process (https://academic.oup.com/nar/article/44/6/2491/2499459).

Source

The part was acquired as synthesized sequences from IDT and Twist Bioscience.

References

Fernandez-Millan, P., Autour, A., Ennifar, E., Westhof, E., & Ryckelynck, M. (2017). Crystal structure and fluorescence properties of the iSpinach aptamer in complex with DFHBI. RNA, 23(12), 1788–1795. https://doi.org/10.1261/rna.063008.117

Neubacher, S., & Hennig, S. (2018). RNA structure and cellular applications of fluorescent light-up Aptamers. Angewandte Chemie International Edition, 58(5), 1266–1279. https://doi.org/10.1002/anie.201806482

Paige, J. S., Wu, K. Y., & Jaffrey, S. R. (2011). RNA mimics of green fluorescent protein. Science, 333(6042), 642–646. https://doi.org/10.1126/science.1207339