Difference between revisions of "Part:BBa K4140025"
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We are developing a tunable therapeutic approach to give the body the ability to produce phenylalanine hydroxylase (PAH) by implementing our designed therapeutic circuit in a cell-based system that produces PAH according to phenylalanine and tyrosine levels as shown in figure 1. In the presence of phenylalanine (Phe), the TyrR will activate tyrP, accordingly tyrP will enhance the production of PAH gene. While the inhibitory effect of the ParoF will not be initiated, thus L7Ae will be expressed, preventing the expression of the dcas12g. | We are developing a tunable therapeutic approach to give the body the ability to produce phenylalanine hydroxylase (PAH) by implementing our designed therapeutic circuit in a cell-based system that produces PAH according to phenylalanine and tyrosine levels as shown in figure 1. In the presence of phenylalanine (Phe), the TyrR will activate tyrP, accordingly tyrP will enhance the production of PAH gene. While the inhibitory effect of the ParoF will not be initiated, thus L7Ae will be expressed, preventing the expression of the dcas12g. | ||
| − | [[File:therap.png|Figure 1. (shows the whole cell-based biosensor.) ]] | + | [[File:therap.png|Right|Figure 1. (shows the whole cell-based biosensor.) ]] |
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Revision as of 17:44, 6 October 2022
Regulatory device
Part Description
Coding sequence for an enzyme that causes phenylalanine to be hydroxylated in order to produce tyrosine. PAH is a hydroxylase that needs biopterin. It's linked to KP-SP ISA secreting peptide signal at the N-terminus of the protein to transmit it from intra cellular to extra cellula
Usage
We are developing a tunable therapeutic approach to give the body the ability to produce phenylalanine hydroxylase (PAH) by implementing our designed therapeutic circuit in a cell-based system that produces PAH according to phenylalanine and tyrosine levels as shown in figure 1. In the presence of phenylalanine (Phe), the TyrR will activate tyrP, accordingly tyrP will enhance the production of PAH gene. While the inhibitory effect of the ParoF will not be initiated, thus L7Ae will be expressed, preventing the expression of the dcas12g.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 820
Illegal PstI site found at 1294
Illegal PstI site found at 2236 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 820
Illegal PstI site found at 1294
Illegal PstI site found at 2236 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1815
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 820
Illegal PstI site found at 1294
Illegal PstI site found at 2236 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 820
Illegal PstI site found at 1294
Illegal PstI site found at 2236
Illegal NgoMIV site found at 1737 - 1000COMPATIBLE WITH RFC[1000]