Difference between revisions of "Part:BBa K4140025"

 
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<partinfo>BBa_K4140026 short</partinfo>
 
<partinfo>BBa_K4140026 short</partinfo>
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==Part Description==
 
Coding sequence for an enzyme that causes phenylalanine to be hydroxylated in order to produce tyrosine. PAH is a hydroxylase that needs biopterin. It's linked to  KP-SP ISA secreting peptide signal  at the N-terminus of the protein to transmit it from intra cellular to extra cellula
 
Coding sequence for an enzyme that causes phenylalanine to be hydroxylated in order to produce tyrosine. PAH is a hydroxylase that needs biopterin. It's linked to  KP-SP ISA secreting peptide signal  at the N-terminus of the protein to transmit it from intra cellular to extra cellula
  
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==Usage==
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We are developing a tunable therapeutic approach to give the body the ability to produce phenylalanine hydroxylase (PAH) by implementing our designed therapeutic circuit in a cell-based system that produces PAH according to phenylalanine and tyrosine levels as shown in figure 1.
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[[File:theap.png|Figure 1. (shows the whole cell-based biosensor in our therapeutic approach. ) ]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:39, 6 October 2022


Regulatory device

Part Description

Coding sequence for an enzyme that causes phenylalanine to be hydroxylated in order to produce tyrosine. PAH is a hydroxylase that needs biopterin. It's linked to KP-SP ISA secreting peptide signal at the N-terminus of the protein to transmit it from intra cellular to extra cellula

Usage

We are developing a tunable therapeutic approach to give the body the ability to produce phenylalanine hydroxylase (PAH) by implementing our designed therapeutic circuit in a cell-based system that produces PAH according to phenylalanine and tyrosine levels as shown in figure 1. Figure 1. (shows the whole cell-based biosensor in our therapeutic approach. )

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 820
    Illegal PstI site found at 1294
    Illegal PstI site found at 2236
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 820
    Illegal PstI site found at 1294
    Illegal PstI site found at 2236
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1815
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 820
    Illegal PstI site found at 1294
    Illegal PstI site found at 2236
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 820
    Illegal PstI site found at 1294
    Illegal PstI site found at 2236
    Illegal NgoMIV site found at 1737
  • 1000
    COMPATIBLE WITH RFC[1000]