Difference between revisions of "Part:BBa K4385015"

 
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<partinfo>BBa_K4385015 short</partinfo>
 
<partinfo>BBa_K4385015 short</partinfo>
  
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The zinc ion sensor part consists of the ZraSR two-component membrane associated sensor kinase system and the PzraP promoter. ZraSR consists of histidine kinase ZraS, cytoplasmic transcription factor ZraR and periplasmic accessory protein ZraP, responsible for sensing the presence of external Zn2+. The ZraSR system regulates the expression of zraP, which encodes a periplasmic zinc-binding protein, in response to high concentrations of extracellular zinc ions; phosphorylated ZraR triggers divergent transcription of zraP and zraSR.
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This part was mainly composed of constitutive promoter J23100, the anchoring protein OmpC-CBP and the heavy metal detoxification protein. Promoter J23100 continuously expressed downstream copper ion surface display proteins to verify the function of outer membrane protein C and lipoprotein of copper ion surface display system. The 6*His Tags were added to this sequence to purify the recombinant protein.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 16:12, 6 October 2022


Copper constitutive surface display

This part was mainly composed of constitutive promoter J23100, the anchoring protein OmpC-CBP and the heavy metal detoxification protein. Promoter J23100 continuously expressed downstream copper ion surface display proteins to verify the function of outer membrane protein C and lipoprotein of copper ion surface display system. The 6*His Tags were added to this sequence to purify the recombinant protein.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 414
    Illegal SpeI site found at 573
    Illegal PstI site found at 921
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 414
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1289
    Illegal NheI site found at 1312
    Illegal SpeI site found at 573
    Illegal PstI site found at 921
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 414
    Illegal BglII site found at 122
    Illegal BglII site found at 1424
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 414
    Illegal SpeI site found at 573
    Illegal PstI site found at 921
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 414
    Illegal SpeI site found at 573
    Illegal PstI site found at 921
    Illegal AgeI site found at 240
  • 1000
    COMPATIBLE WITH RFC[1000]